Types of Culture Callus Culture: Callus tissue means an unorganised proliferative mass of cells produced from isolated plant cells, tissues or organs when grown aseptically on artificial nutrient medium in glass vials under controlled experimental conditions. Principles of Callus Culture: Aseptic preparation of plant material, Selection of suitable nutrient medium supplemented with appropriate ratio of auxins and cytokinins or only appropriate auxin, and Incubatif culture under controlled physical condition. Requirement:- Carrot, Petri plates, Flasks, Scalpel, Incubator, Teepol, ethanol, Sodium hypochlorite, D/W, MS media Protocol of Callus Culture: Callus tissue can be induced from different plant tissues of many plant species. Carrot is a highly standardized material. (1) A fresh tap root of carrot is taken and washed thoroughly under running tap water to remove all surface detritus (2) The tap root is then di...
